ictaluri was very pathogenic on striped catfish P. Decreased of hematocrit and hemoglobin values of all tread group were statistically significant different (P4 cfu/mL. Histopathology showed hydropic degeneration, fatty degeneration, hemorrhage and necrosis in the liver, melano macrophage center (MMC) and necrosis in the kidneys, hemorrhage, and inflammatory cell infiltrates were also found in the kidneys and brain. Clinical signs and pathology anatomy of co-infection fish showed vertical swim, petechial hemorrhage in the skin, dropsy, ascites in the abdominal cavity, pale liver and the kidney was dark red. Internal organ sample were taken on the 5th day for histopatologic test while blood sample was on the 1st, 3rd, and 5th day after infection. External organs of fish (skin and abdomen) and internal organs (liver, kidney, and brain) were examined macroscopicly and microscopicly.

Thirty individu of five group fishes, 6–10 g in body weight, injected intraperitoneally with 0,1 mL of bacteria suspension of 102 cfu/mL 104 cfu/mL 106 cfu/mL 108 cfu/mL 1010 cfu/mL and PBS as control, were culture in 18 of 60×40×45 cm3 aquarium for seven days. This reaserch was aimed to determine pathogenicity of local isolate E. One of major problem of striped catfish Pangasionodon hypophthalmus culture is enteric septicemia of catfish (ESC), bacterial disease of Edwardsiella ictaluri, caused of more than 50% of mortalities. Histopathological examination revealed diffuse severe congestion in blood vessels and several degenerative and necrotic changes inconcurrent with positive antigenic staining by immunohistochemistry. Postmortem lesions were recorded as exophthalmia, bloody ascitis, hemorrhagic kidney and distended gallbladder, meningio-encephalitis, hemorrhagic vent, and petechial hemorrhages on viscera. Daily collection of five moribund fish for necropsy and tissue specimens collection from hepatopancreas, spleen, posterior kidney, gills, brain, heart, and intestine for histopathological and immunohistochemical examination. ictaluri by slide agglutination using anti-PH0744 serum. The fish were observed for clinical signs, with daily collection of dead fish, and isolation of bacteria from the posterior kidney was performed and confirmed to be E. The fish were injected with the isolate intraperitoneally with a dose of 5.1 × 105 cfu/fish, while the control fish were injected with sterile phosphate buffered saline. Infection experiments were performed using 44 healthy cultured ayu fingerlings using E. In this study, histopathological findings and immunohistochemical localization of the bacteria following experimental infection of ayu were discussed. In recent decades, several mass mortalities were recorded in riverine ayu (Plecoglossus altivelis) in Tokyo Metropolis, Hiroshima Prefecture, and Yamaguchi Prefecture, Japan in these outbreaks, microbiological and pathological examinations revealed Edwardsiella ictaluri as the causative agent.

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